基質(zhì)細(xì)胞衍生因子1(SDF1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Stromal Cell Derived Factor 1 (SDF1)
CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
- 編號UKSA122Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.057ng/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 2840 ¥ 4734 ¥ 8521 ¥ 16569 ¥ 28404
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對基質(zhì)細(xì)胞衍生因子1(SDF1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA122Hu01 | 基質(zhì)細(xì)胞衍生因子1(SDF1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA122Hu02 | 基質(zhì)細(xì)胞衍生因子1(SDF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA122Hu62 | 基質(zhì)細(xì)胞衍生因子1(SDF1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA122Hu01 | 基質(zhì)細(xì)胞衍生因子1(SDF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA122Hu02 | 基質(zhì)細(xì)胞衍生因子1(SDF1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA122Hu63 | 基質(zhì)細(xì)胞衍生因子1(SDF1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA122Hu61 | 基質(zhì)細(xì)胞衍生因子1(SDF1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA122Hu02 | 基質(zhì)細(xì)胞衍生因子1(SDF1)多克隆抗體 | WB |
| UPAA122Hu01 | 基質(zhì)細(xì)胞衍生因子1(SDF1)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| ULAA122Hu71 | 基質(zhì)細(xì)胞衍生因子1(SDF1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA122Hu21 | 基質(zhì)細(xì)胞衍生因子1(SDF1)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA122Hu23 | 基質(zhì)細(xì)胞衍生因子1(SDF1)單克隆抗體 | ICC/IF |
| UMAA122Hu22 | 基質(zhì)細(xì)胞衍生因子1(SDF1)單克隆抗體 | ICC/IF |
| USEA122Hu | 基質(zhì)細(xì)胞衍生因子1(SDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA122Hu | 基質(zhì)細(xì)胞衍生因子1(SDF1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA122Hu01 | 基質(zhì)細(xì)胞衍生因子1(SDF1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Graefe's Archive for Clinical and Experimental Ophthalmology | Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells [PubMed: 21655971] |
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| Experimental Eye Research | Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells [Pubmed: 24780853] |
| Atherosclerosis | The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial [Pubmed:24530788] |
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| Tissue Engineering Part A | Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat [Pubmed:24814095] |
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| Journal of Stroke and Cerebrovascular Diseases | Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients [Pubmed:25444027] |
| Pharmacological Reports | The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders [Pubmed:25560580] |
| In Vitro Cellular & Developmental Biology - Animal | Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells [Pubmed:25636237] |
| Pharmacological Reports | Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder [PubMed: 26481549] |
| Biochem Biophys Res Commun | Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats [PubMed: 26392311] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats [PubMed: 26396666] |
| Medicine (Baltimore) | A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion [PubMed: 26632727] |
| Pharmacological Reports | A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity [Pubmed:27208878] |
| Frontiers in Psychiatry | Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. [pubmed:28149283] |
| European?Journal?of?Applied?Physiology | High-intensity?Interval?training?enhances?mobilization/functionality?of?endothelial?progenitor?cellsand?depressed?shedding?of?vascular?endothelial?cells?undergoing hypoxia. [pubmed:27761657] |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs [pubmed:29233725] |
| International Journal of?Clinical?and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| BioMed Research International. | c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer [pubmed:28573141] |
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| International Journal of Molecular?Medicine | Low?intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1?mediated SDF?1 expression [Pubmed:29620151] |
| Cardiovascular Drugs and Therapy | β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats [Pubmed:29752864] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation [Doi: 10.1016/j.jcyt.2018.07.007] |
| ACS?Applied?Materials?&?Interfaces | Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration [Pubmed: 30777748] |
| World?Journal of?Stem?Cells | Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells [Pubmed: 30949297] |
| Stem Cells International | Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells [Pubmed: 31827537] |
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| Journal of tissue engineering and regenerative medicine | Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4 [Pubmed: 32633015] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| pharmaceuticals | bFGF and SDF-1|á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts [33800631] |
| Microb Biotechnol | Combination of an engineered Lactococcus?lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice [34310856] |
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