甲狀旁腺激素(PTH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Parathyroid Hormone (PTH)
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin
- 編號(hào)UKSA866Ga01
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.070ng/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3566 ¥ 5943 ¥ 10697 ¥ 20801 ¥ 35658
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)甲狀旁腺激素(PTH)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA866Ga01 | 甲狀旁腺激素(PTH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA866Ga01 | 甲狀旁腺激素(PTH)多克隆抗體 | WB |
| ULAA866Ga71 | 甲狀旁腺激素(PTH)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA866Ga81 | 甲狀旁腺激素(PTH)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UKSA866Ga01 | 甲狀旁腺激素(PTH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biological trace element research | Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet [PubMed: 19915804] |
| Basic nutritional investigation | Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice [ScienceDirect: S0899900709004808] |
| The Korean Journal of Nutrition | Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats [KoreaMed: source] |
| Acta Physiologica Hungarica | Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters [Pubmed: 22173025] |
| Toxicology and Industrial Health | The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat [PubMed: 22782709] |
| Indian Journal of Experimental Biology | Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. [PubMed: 22439438] |
| Plos one | Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? [PubMed: PMC3679169] |
| Nutr Hosp. | Effect of the “protein diet” and bone tissue. [Pubmed: 24483972] |
| Clinical Cancer Research | Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo [Aacrjournals: Source] |
| Nutricion Hospitalaria | Effect of the “protein diet” and bone tissue [Aulamedica: Source] |
| Academic Journal | Effect of whole body vibration on healthy rat plasma parameters [Ebscohost: Source] |
| J Proteome Res. | Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets [Pubmed:25051233] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370] |
| J Nutr Sci Vitaminol (Tokyo) | The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats [PubMed: 26639847] |
| The Korean Society of Pharmacognosy | Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat [Article: Hksobf_2015_V46N2_167] |
| J?Bone?Miner Res | Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats [Pubmed:26175082] |
| 7 | Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. [pubmed:28218908] |
| PLOS?ONE | Prostaglandin-E2?Mediated?Increase?in?Calcium?and?Phosphate?Excretion?in a?Mouse?Model?ofDistal?Nephron?Salt?Wasting. [pubmed:27442254] |
| International Journal of Advanced Research in Biological Sciences | Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat [10.22192/ijarbs.2017.04.04.018] |
| American Journal of Physiology-Renal?Physiology | Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice [Pubmed:29363324] |
| Tropical?Animal?Health?and Production | Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism [Pubmed:29931604] |
| International Immunopharmacology | CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts [Pubmed: 31679847] |
| ENDOCRINOLOGY | A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female Rats [Pubmed: 32852547] |
| Kidney International | Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts. [] |
| PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES [] | |
| Oxid Med Cell Longev | Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats [34373766] |
| Gene Therapy | Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector [34599290] |
| Cells | Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury [Pubmed:35456008] |
| African Health Sciences | Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease [] |
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