肝素酶(HPSE)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Heparanase (HPSE)
HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;
- 編號(hào)UKSA711Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍31.2-2000pg/mL
- 可適靈敏度12.3pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)肝素酶(HPA)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA711Hu04 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA711Hu02 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA711Hu04 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA711Hu01 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA711Hu61 | 肝素酶(HPSE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA711Hu61 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA711Hu01 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA711Hu03 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA711Hu21 | 肝素酶(HPSE)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA711Hu04 | 肝素酶(HPSE)多克隆抗體 | IHC |
| UPAA711Hu02 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA711Hu01 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA711Hu03 | 肝素酶(HPSE)多克隆抗體 | IHC |
| UFAA711Hu01 | 抗肝素酶(HPSE)多克隆抗體 | FCM |
| UFAA711Hu03 | 抗肝素酶(HPSE)多克隆抗體 | FCM |
| ULAA711Hu71 | 肝素酶(HPSE)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA711Hu23 | 肝素酶(HPSE)單克隆抗體 | ICC/IF |
| UMAA711Hu22 | 肝素酶(HPSE)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA711Hu21 | 肝素酶(HPSE)單克隆抗體 | WB; ICC/IF |
| USEA711Hu | 肝素酶(HPSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA711Hu | 肝素酶(HPSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA711Hu | 肝素酶(HPSE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA711Hu01 | 肝素酶(HPSE)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Plos one | PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source] |
| Int J Clin Exp Pathol | Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183] |
| Tumour Biology | PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84] |
| Renal Failure | Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624] |
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