細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Cytochrome P450 2E1 (CYP2E1)
CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1
- 編號(hào)UKSA988Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.064ng/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3694 ¥ 6156 ¥ 11081 ¥ 21546 ¥ 36936
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)細(xì)胞色素P450家族成員2E1(CYP2E1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA988Ra01 | 細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA988Ra02 | 細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA988Ra02 | 細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體 | WB; IHC |
| UPAA988Ra01 | 細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體 | WB; IHC; ICC/IF |
| ULAA988Ra71 | 細(xì)胞色素P450家族成員2E1(CYP2E1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA988Ra21 | 細(xì)胞色素P450家族成員2E1(CYP2E1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA988Ra | 細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA988Ra | 細(xì)胞色素P450家族成員2E1(CYP2E1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA988Ra01 | 細(xì)胞色素P450家族成員2E1(CYP2E1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| BMC Complementary and Alternative Medicine | Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats [PubMed: PMC3605171] |
| Evidence-Based Complementary Alternative Medicine | Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats [PubMed: PMC3749608] |
| BMC Complementary and Alternative Medicine | CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat [PubMed: PMC3519517] |
| Asian Journal of Biochemical and Pharmaceutical Research | Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source] |
| The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats [Pubmed:24701154] |
| Journal of Functional Foods | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo [publication:307092820] |
| CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLA | Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats. [59f7c85aaca272607e2d8d7a] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
| Nutrients | Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages [Pubmed: 31935882] |
| Antioxidants | Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress [34573017] |
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