25-羥基維生素D3(HVD3)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for 25-Hydroxyvitamin D3 (HVD3)
25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
- 編號UKSA915Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍2.47-200ng/mL
- 可適靈敏度0.91ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 4298 ¥ 7164 ¥ 12895 ¥ 25074 ¥ 42984
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對25-羥基維生素D3(HVD3)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| UCPA915Ge11 | 25-羥基維生素D3(HVD3)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| UCPA915Ge21 | 25-羥基維生素D3(HVD3)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| UPAA915Ge01 | 25-羥基維生素D3(HVD3)多克隆抗體 | ELISA |
| ULAA915Ge71 | 25-羥基維生素D3(HVD3)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA915Ge22 | 25-羥基維生素D3(HVD3)單克隆抗體 | ELISA |
| UMAA915Ge21 | 25-羥基維生素D3(HVD3)單克隆抗體 | ELISA |
| UCEA915Ge | 25-羥基維生素D3(HVD3)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA915Ge | 25-羥基維生素D3(HVD3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA915Ge11 | 25-羥基維生素D3(HVD3)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Allergy & Clinical Immunology | Decreased serum LL-37 and vitamin D3 levels in atopic dermatitis: relationship between IL-31 and oncostatin M [Wiley: source] |
| Tianjin Med J | 成人哮喘血清維生素D 水平與肺功能相關因素分析 [Jwk_Tjyy: Cn] |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women [S1756464617301561] |
| J Nutr Biochem. | Chronic maternal calcium and 25-hydroxyvitamin D deficiency in Wistar rats programs abnormal hepatic gene expression leading to hepatic steatosis in female offspring. [pubmed:28219837] |
| ?in vivo | Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level [pubmed:28064234] |
| Asia pacific allergy | Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis. [pubmed:29094019] |
| Biomed?Research?International? | Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar Rats [Pubmed:29850540] |
| Clin Implant Dent Relat Res. | Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases [Pubmed: 31407857] |
| JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in?… [Pubmed: 33065276] |
| The Journal of Obstetrics and Gynecology of India | The Effects of Follicular Fluid 25 (OH) D Concentration on Intrafollicular Estradiol Level, Oocyte Quality, and Fertilization Rate in Women Who Underwent IVF Program [] |
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