核因子κB受體激活因子配體(RANkL)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine
- 編號(hào)USCA855Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)2h, 40min
- 檢測(cè)范圍2.74-2,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.96pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1915 ¥ 2736 ¥ 12312 ¥ 23256 ¥ 191520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)核因子κB受體激活因子配體(RANkL)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的核因子κB受體激活因子配體(RANkL)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-90 | 86 |
| EDTA plasma(n=5) | 78-101 | 83 |
| heparin plasma(n=5) | 94-102 | 97 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的核因子κB受體激活因子配體(RANkL)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中核因子κB受體激活因子配體(RANkL)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 98-105% | 84-93% | 78-98% |
| EDTA plasma(n=5) | 81-102% | 86-94% | 94-104% | 80-94% |
| heparin plasma(n=5) | 85-93% | 88-102% | 86-96% | 82-90% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100μL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將核因子κB受體激活因子配體(RANkL)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的核因子κB受體激活因子配體(RANkL)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的核因子κB受體激活因子配體(RANkL)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的核因子κB受體激活因子配體(RANkL)呈正相關(guān)。用化學(xué)發(fā)光儀測(cè)定相對(duì)光單位（RLU），計(jì)算樣品濃度。
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| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA855Ra61 | 核因子κB受體激活因子配體(RANkL)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA855Ra01 | 核因子κB受體激活因子配體(RANkL)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA855Ra01 | 核因子κB受體激活因子配體(RANkL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA855Ra01 | 核因子κB受體激活因子配體(RANkL)多克隆抗體 | WB |
| UMAA855Ra24 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA855Ra26 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB |
| UMAA855Ra22 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; ICC/IF |
| UMAA855Ra23 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB; ICC/IF |
| UMAA855Ra21 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | WB |
| UMAA855Ra25 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | ICC/IF |
| UMAA855Ra27 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | ICC/IF |
| UMAA855Ra28 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | ICC/IF |
| UMAA855Ra29 | 核因子κB受體激活因子配體(RANkL)單克隆抗體 | ICC/IF |
| ULAA855Ra76 | 核因子κB受體激活因子配體(RANkL)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA855Ra | 核因子κB受體激活因子配體(RANkL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA855Ra | 核因子κB受體激活因子配體(RANkL)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA855Ra | 核因子κB受體激活因子配體(RANkL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA855Ra01 | 核因子κB受體激活因子配體(RANkL)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA855Ra01 | 核因子κB受體激活因子配體(RANkL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Archives of Oral Biology | Compressive stress induced the up-regulation of M-CSF, RANKL, TNF-α expression and the down-regulation of OPG expression in PDL cells via the integrin-FAK pathway [ScienceDirect: S0003996912003998] |
| PLOS One | Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone Erosions [PubMed: PMC3483160] |
| Arthritis Research&Therapy | Regulatory effect of calcineurin inhibitor, tacrolimus, on IL-6/sIL-6R-mediated RANKL expression through JAK2-STAT3-SOCS3 signaling pathway in fibroblast-like synoviocytes [PubMed: PMC3672788] |
| International Journal of Oral and Maxillofacial Surgery | The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model [Pubmed: 23522850] |
| AGE | Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells [Pubmed: 24687841] |
| Food Science & Nutrition | Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 [Onlinelibrary: fsn3.109] |
| Food Sci Nutr. | Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 [Pubmed:Pmc4221832] |
| Journal of Investigative Surgery | Effect of Nicotine on RANKL and OPG and Bone Mineral Density [Pubmed:24830656] |
| Int J Mol Med. | Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma … [Pubmed:25270538] |
| Clinical and Experimental Pharmacology and Physiology | Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats [Pubmed:25424914] |
| Int J Clin Exp Pathol | A novel model of bisphosphonate-related osteonecrosis of the jaw in rats [PubMed: 26191212] |
| oncotarget | MicroRNA-106b inhibits osteoclastogenesis and osteolysis by targeting RANKL in giant cell tumor of bone [PubMed: 26053181] |
| European Journal of Clinical Investigation | Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke [PubMed: 25845543] |
| Scienze Biomediche | Effetti del laser a bassa dose su cellule osteoblastiche [INCERTIPARENTI_SERENA_TESI.pdf] |
| Nutrition. | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. [pubmed:27908549] |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228] |
| Epilepsy?Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501] |
| Int Endod J.? | Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide. [pubmed:28333374] |
| Sci Rep.? | Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. [pubmed:28303909] |
| Sci Rep. | Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. [pubmed:28139685] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| BMJ Journals | SAT0003?The Role of 12/15-Lipoxygenase in The Pathogenesis of Osteoporosis in Mice [content:75] |
| Japanese Society for Bone and Mineral Research | Allogeneic yet?major histocompatibility complex?matched bone marrow transplantation in?mice results in?an?impairment of?osteoblasts and?a?significantly reduced trabecular bone [article:10.1007] |
| ONCOLOGY LETTERS | Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause [ol.2017.6596] |
| Journal of?Clinical?Periodontology? | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908] |
| Journal of Functional Foods | Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats [10.1016:j.jff.2018.04.048] |
| International Immunopharmacology | Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment [Pubmed:30015235] |
| European?Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468] |
| Journal of?Bone?and?Mineral?Metabolism | Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced?… [Pubmed:28766138] |
| Experimental and Therapeutic Medicine | Liraglutide exerts a bone?protective effect in ovariectomized rats with streptozotocin?induced diabetes by inhibiting osteoclastogenesis [Pubmed:29805533] |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342] |
| Journal of?Periodontal?Research | Improved RANKL expression and osteoclastogenesis induction of CD27+ CD38? memory B cells: A link between B cells and alveolar bone damage in periodontitis [Pubmed: 30346027] |
| Bone?Research | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992] |
| Journal of International Dental and Medical Research | Alendronate Effect in RANKL Concentration at Ovariectomized Sprague Dawley Rat. [] |
| Bone | Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process [Pubmed: 30797058] |
| Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology | Evaluation of therapeutic effects of teriparatide in a rat model of zoledronic acid-induced bisphosphonate-related osteonecrosis [] |
| Rheumatology International | Comparison of RANKL expression, inflammatory markers, and cardiovascular risk in patients with acute coronary syndrome with and without rheumatoid arthritis [Pubmed: 31297563] |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939] |
| Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
| BIOSCIENCE REPORTS | IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway [Pubmed: 32347300] |
| Journal of Ethnopharmacology | Anti-osteoporosis effect of Semen Cuscutae in ovariectomized mice through inhibition of bone resorption by osteoclasts [34801609] |
| Br J Haematol | Inducible T‐cell co‐stimulator (ICOS) and ICOS ligand are novel players in the multiple‐myeloma microenvironment [Pubmed:34954822] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007] |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK?… [Pubmed:35660352] |
| Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood [] |
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