肝細胞生長因子(HGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- 編號USCA047Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長2h, 40min
- 檢測范圍1.37-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于0.58pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1915 ¥ 2736 ¥ 12312 ¥ 23256 ¥ 191520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測肝細胞生長因子(HGF)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的肝細胞生長因子(HGF)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-104 | 93 |
| EDTA plasma(n=5) | 79-93 | 89 |
| heparin plasma(n=5) | 78-93 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的肝細胞生長因子(HGF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中肝細胞生長因子(HGF)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 86-101% | 79-103% | 97-105% |
| EDTA plasma(n=5) | 80-103% | 84-95% | 79-93% | 78-95% |
| heparin plasma(n=5) | 78-97% | 90-98% | 79-101% | 85-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將肝細胞生長因子(HGF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的肝細胞生長因子(HGF)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的肝細胞生長因子(HGF)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的肝細胞生長因子(HGF)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA047Rb01 | 肝細胞生長因子(HGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA047Rb51 | 肝細胞生長因子(HGF)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA047Rb21 | 肝細胞生長因子(HGF)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA047Rb | 肝細胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA047Rb | 肝細胞生長因子(HGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA047Rb | 肝細胞生長因子(HGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998] |
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| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963] |
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| Nature?Communications | Pulmonary pericytes regulate lung morphogenesis [Pubmed:29934496] |
| Experimental And Molecular?Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke?… [Pubmed:29650950] |
| Oncology?Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro [Pubmed:29375714] |
| Bulletin?of Experimental?Biology?and?Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats [Pubmed:29796817] |
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