內(nèi)皮素1(EDN1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- 編號UCCA482Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍3.9-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于1.4pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1764 ¥ 2520 ¥ 11340 ¥ 21420 ¥ 176400
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測內(nèi)皮素1(EDN1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的內(nèi)皮素1(EDN1)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-91 | 83 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 92-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的內(nèi)皮素1(EDN1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中內(nèi)皮素1(EDN1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 96-104% | 98-105% | 83-104% |
| EDTA plasma(n=5) | 96-104% | 83-98% | 90-99% | 78-93% |
| heparin plasma(n=5) | 88-97% | 98-105% | 78-104% | 93-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將內(nèi)皮素1(EDN1)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原（標(biāo)準(zhǔn)品或樣本），待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號。發(fā)射光強(qiáng)度和樣品中的內(nèi)皮素1(EDN1)呈負(fù)相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA482Hu01 | 內(nèi)皮素1(EDN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA482Hu11 | 內(nèi)皮素1(EDN1)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA482Hu21 | 內(nèi)皮素1(EDN1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA482Hu08 | 內(nèi)皮素1(EDN1)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA482Hu01 | 內(nèi)皮素1(EDN1)多克隆抗體 | IHC |
| ULAA482Hu81 | 內(nèi)皮素1(EDN1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA482Hu71 | 內(nèi)皮素1(EDN1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA482Hu23 | 內(nèi)皮素1(EDN1)單克隆抗體 | ICC/IF |
| UMAA482Hu22 | 內(nèi)皮素1(EDN1)單克隆抗體 | IHC |
| UMAA482Hu21 | 內(nèi)皮素1(EDN1)單克隆抗體 | IHC |
| ULAA482Hu72 | 內(nèi)皮素1(EDN1)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UIEA482Hu | 內(nèi)皮素1(EDN1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA482Hu | 內(nèi)皮素1(EDN1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA482Hu | 內(nèi)皮素1(EDN1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA482Hu | 內(nèi)皮素1(EDN1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA482Hu11 | 內(nèi)皮素1(EDN1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source] |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
| 36 | Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 [PubMed: PMC3695508] |
| Clinics (Sao Paulo). | Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice [Pubmed: 24212843] |
| Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829] |
| Transpl Int. | One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys [Pubmed:24977654] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337] |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880] |
| Journal of Surgical Research | Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning [Sciencedirect:S0022480414004909] |
| Pharmaceutical Biology | The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil [PubMed: 26023989] |
| A Periodical of the Hungarian Academy of Sciences | The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients [PubMed: 26690031] |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880] |
| Human & Experimental Toxicology | An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury [PubMed: 26078283] |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [science:article] |
| Transplant? proc | Cardiovascular?Risk Factors?and?Markers?of?Atherosclerosis?in?Stable?Kidney TransplantRecipients. [pubmed:27496444] |
| Biomed Journal | Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. [pubmed:27884380] |
| International Journal of Advanced Research in Biological Sciences | Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose [pdfcopy:42552] |
| Journal of Drug Metabolisn& Toxicology | Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose [587e5b6508aed3826af45e2d.pdf] |
| Polish?Journal of Veterinary?Sciences | Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema [pubmed:27760029] |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [article:S1872-9312(16)30085-0] |
| Journal of Molecular Biomarkers J & Diagnosis | Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses [DOI: 10.4172/2155-9929.1000318] |
| A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. [AN=123819331] | |
| Journal of Functional Foods | Antihypertensive effects of?Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033] |
| Radiation?Research | Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute?… [Pubmed:29809108] |
| Scandinavian Journal of?Clinical?and Laboratory Investigation | Isolated kidney perfusion: the influence of pulsatile flow [Pubmed:29301417] |
| Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension?… [Pubmed: 30359461] |
| Journal of Pharmacological?Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783] |
| Molecular Nutrition &?Food?Research | Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight?… [Pubmed: 30570214] |
| Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159] |
| Eurasian Journal of Clinical Sciences | APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan [] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats [Pubmed: 33133215] |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534] |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti?… [34736077] |
| J Ethnopharmacol | Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells [34752899] |
| J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats [34343648] |
| Optical Technologies for Biology and Medicine | Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency [] |
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