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內(nèi)脂素(VF)多克隆抗體

Polyclonal Antibody to Visfatin (VF)

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

編號UPAA638Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價-
Ig類型 IgG
純化方式抗原特異性親和純化
標記物無標記物
免疫原 RPA638Hu01-內(nèi)脂素(VF)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/ml
且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
應用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Esophagus Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human VF Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human VF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human VF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human VF Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant VF, Human.
Western Blot; Sample: Porcine Liver lysate; Primary Ab: 1μg/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: Porcine Heart lysate; Primary Ab: 1μg/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: Porcine Stomach lysate; Primary Ab: 1μg/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: 293T cell lysate; Primary Ab: 1μg/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: VF knockout 293T cell lysate;
Predicted MW: 55kd
Observed MW: 55kd
Primary Ab: 1μg/ml Rabbit Anti-Human VF Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

該抗體是針對VF的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別VF。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務

抗體標記定制服務
 Protein-A/G純化柱
 細胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務
 磷酸化抗體定制服務
 免疫印跡(WB)實驗服務
 免疫組織化學(IHC)實驗服務
 免疫細胞化學(ICC)實驗服務
 流式細胞術(shù)(FCM)實驗服務
 免疫沉淀(IP)實驗服務
 免疫熒光(IF)實驗服務
 緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標記試劑盒
 實時熒光定量PCR實驗服務

參考文獻

雜志	參考文獻
Brazilian Journal of Biomoricity	EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN [Brjb: source]
Diabetes Research and Clinical Practice	The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance [ScienceDirect: S0168822711002956]
J Physiol Pharmacol.	Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. [Pubmed: 24304571]
Endocr J.	CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Exp Ther Med.	Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome [Pubmed:Pmc3991522]
Eur J Cancer Prev.	Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. [Pubmed:25055182]
Genome	Visfatin ?948G/T?and resistin ?420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases [Nrcresearchpress:Source]
PLOS ONE	Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991]
Alcoholism and Drug Addiction	Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males [Science: Article]
Cytokine	Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies [PubMed: 26539806]
Genome	Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications [PubMed: 25120107]
Acta Histochemica	Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
Endocrine, Metabolic & Immune Disorders-Drug Targets	Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. [pubmed:28699485]
Endocrine?Connections	Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery [Pubmed:30012648]
Bioscience Reports	Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats [Pubmed:29784871]
Contraception	Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive [Pubmed:29665358]
Cranio?-?Journal?of?Craniomandibular?Practice	Analysis of synovial fluid visfatin level in temporomandibular joint disorders [Pubmed:29385907]
Nutrire	Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children [10.1186:s41110-018-0060-7]
DNA?and?Cell?Biology	Transcriptional Regulation of?NAMPT?Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes [Pubmed: 30615481]
Jundishapur?Journal of?Chronic?Disease?Care	The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men []
Biomarkers in Heart Failure and Associated Diseases	Adipokines as Biomarkers in Health and Disease []
Journal of Translational Medicine	Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern [Pubmed: 31533725]
clinical neurology and neurosurgery	Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618]
The Horizon of Medical Sciences	Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females []
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY	Different Ratios of Corn and Coconut Oil Blends in High?\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats []

留言咨詢

編號	適用物種：Homo sapiens (Human，人)	應用(僅供研究使用，不用于臨床診斷！)
URPA638Hu01	內(nèi)脂素(VF)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UCPA638Hu21	內(nèi)脂素(VF)卵白蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
UPAA638Hu01	內(nèi)脂素(VF)多克隆抗體	WB; IHC
UPAA638Hu08	內(nèi)脂素(VF)多克隆抗體	WB; IHC; ICC; IP.
UFAA638Hu01	抗內(nèi)脂素(VF)多克隆抗體	FCM
UMAA638Hu22	內(nèi)脂素(VF)單克隆抗體	WB
UMAA638Hu27	內(nèi)脂素(VF)單克隆抗體	IHC
UMAA638Hu29	內(nèi)脂素(VF)單克隆抗體	IHC; ICC/IF
UMAA638Hu21	內(nèi)脂素(VF)單克隆抗體	WB
UMAA638Hu23	內(nèi)脂素(VF)單克隆抗體	WB; IHC
UMAA638Hu24	內(nèi)脂素(VF)單克隆抗體	WB; IHC
UMAA638Hu25	內(nèi)脂素(VF)單克隆抗體	WB; IHC
UMAA638Hu26	內(nèi)脂素(VF)單克隆抗體	WB; FCM
UFAA638Hu04	抗內(nèi)脂素(VF)單克隆抗體	FCM
UFAA638Hu02	抗內(nèi)脂素(VF)單克隆抗體	FCM
UFAA638Hu06	抗內(nèi)脂素(VF)單克隆抗體	FCM
UFAA638Hu08	抗內(nèi)脂素(VF)單克隆抗體	FCM
USEA638Hu	內(nèi)脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UMEA638Hu	內(nèi)脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
UCEA638Hu	內(nèi)脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA638Hu	內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA638Hu11	內(nèi)脂素(VF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".