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波形蛋白(VIM)單克隆抗體

Monoclonal Antibody to Vimentin (VIM)

編號UMAB040Hu22
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號)C12
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB040Hu01-波形蛋白(VIM)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/mL
且適物種Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價(jià)格 ¥ 315 735 ¥ 735 ¥ 1050 ¥ 2625 ¥ 10500
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

FITC staining on IF; Samples: Human MCF7 Cells; Primary Ab: 40μg/ml Mouse Anti-Human VIM Antibody Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Vector Red staining on IHC-P;
Samples: Human Testis Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Monoclonal Antibody
FITC and CY3 staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 40μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
Cy3 staining on IF;
Sample: Primary Canine Bladder Stromal Fibroblasts cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16)
Cy3 staining on IF;
Sample: Primary Rabbit Neonatal Dermal Fibroblasts cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16)
Cy3 staining on IF;
Sample: Primary Rabbit Corneal Stromal cells cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16)
FITC staining on IF;
Sample: Primary Rabbit Myocardial Cells cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)

Figure. Western Blot; Sample: Recombinant VIM, Human.
Western Blot; Sample: Rat Heart lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對VIM的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別VIM。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-40μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Cancer?Epidemiology,?Biomarkers?& Prevention	Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367]
Scientific Reports	Glyoxalase?1-knockdown?in?human?aortic?endothelial?cells - effect on the?proteome?andendothelial?function?estimates. [pubmed:27898103]
Journal of?Proteomics	HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555]
Journal of Biological Chemistry	Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328]
American Journal of Transplantation	Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155]
Lung	Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750]
Cell Biol Toxicol	ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221]
CELL BIOLOGY AND TOXICOLOGY	Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996]
	Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women []
	Advanced glycation end-products associate with podocytopathy in type II diabetic patients []
	Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… []

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編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB040Hu02	波形蛋白(VIM)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPB040Hu01	波形蛋白(VIM)活性蛋白	Cell?culture;?Activity?Assays.
URPB040Hu01	波形蛋白(VIM)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UEPB040Hu61	波形蛋白(VIM)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UEPB040Hu62	波形蛋白(VIM)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAB040Hu02	波形蛋白(VIM)多克隆抗體	IHC; ICC/IF
UPAB040Hu01	波形蛋白(VIM)多克隆抗體	WB; IHC; ICC/IF
ULAB040Hu71	波形蛋白(VIM)多克隆抗體(生物素標(biāo)記)	WB
UMAB040Hu24	波形蛋白(VIM)單克隆抗體	WB; IHC; ICC/IF
UMAB040Hu25	波形蛋白(VIM)單克隆抗體	WB; IHC; ICC/IF
UMAB040Hu21	波形蛋白(VIM)單克隆抗體	WB; IHC
UMAB040Hu23	波形蛋白(VIM)單克隆抗體	WB; IHC; ICC/IF
UMAB040Hu27	波形蛋白(VIM)單克隆抗體	WB; IHC; ICC/IF
UMAB040Hu22	波形蛋白(VIM)單克隆抗體	WB; IHC; ICC/IF
UFAB040Hu02	抗波形蛋白(VIM)單克隆抗體	FCM
UFAB040Hu06	抗波形蛋白(VIM)單克隆抗體	FCM
UFAB040Hu04	抗波形蛋白(VIM)單克隆抗體	FCM
UFAB040Hu08	抗波形蛋白(VIM)單克隆抗體	FCM
USEB040Hu	波形蛋白(VIM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB040Hu	波形蛋白(VIM)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSB040Hu01	波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".