基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體
Monoclonal Antibody to Matrix Metalloproteinase 13 (MMP13)
CLG3; Collagenase 3
- 編號UMAA099Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA099Hu01-基質(zhì)金屬蛋白酶13(MMP13)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種-
- 應用WB
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 302 ¥ 706 ¥ 1008 ¥ 2520 ¥ 10080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate Primary Ab: 2μg/ml Mouse Anti-Human MMP13 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對MMP13的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別MMP13。
用法
Western blotting: 0.01-2μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA099Hu01 | 基質(zhì)金屬蛋白酶13(MMP13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA099Hu02 | 基質(zhì)金屬蛋白酶13(MMP13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA099Hu02 | 基質(zhì)金屬蛋白酶13(MMP13)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA099Hu01 | 基質(zhì)金屬蛋白酶13(MMP13)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA099Hu01 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | WB; IHC |
| UPAA099Hu02 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | WB |
| UFAA099Hu01 | 抗基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | FCM |
| ULAA099Hu71 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體(生物素標記) | WB |
| UMAA099Hu21 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | IHC; ICC/IF |
| UMAA099Hu23 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | IHC |
| UMAA099Hu24 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | IHC |
| UMAA099Hu25 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | IHC |
| UMAA099Hu27 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA099Hu29 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | ICC/IF |
| UMAA099Hu22 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB |
| UHEA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source] |
| Arthritis & Rheumatism | Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice [Wiley: source] |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Arthritis Rheumatol | Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. [Pubmed: 24578232] |
| Arthritis & Rheumatology | Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression [Onlinelibrary: art.38576] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Arthritis & Rheumatol | Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis [Pubmed:24578232] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Cellular Physiology and Biochemistry | Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering [Pubmed:25632940] |
| Osteoarthritis and Cartilage | Depletion of gangliosides enhances cartilage degradation in mice [Sciencedirect:S1063458413010364] |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310] |
| PLoS One | Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression [PubMed: 26474296] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496] |
| Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds [pubmed:27821864] |
| International Journal of Contemporary Medical Research | Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma [uploads:7] |
| Scientific Reports | Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption. [pubmed:27640363] |
| Photodermatol Photoimmunol Photomed. | Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice [pubmed:27995657] |
| Molecular Medicine Reports | Icariin inhibits MMP?1, MMP?3 and MMP?13 expression through MAPK pathways in IL?1β?stimulated SW1353 chondrosarcoma cells [pubmed:28447732] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057] |
| BMC Musculoskeletal Disorders | Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture [pubmed:29149841] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Alexandria Journal of Medicine | L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats [10.1016:j.ajme.2016.03.002] |
| National Journal of Integrated Research in Medicine | Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma. [AN=123116960] |
| Molecular?Medicine?Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits [Pubmed:29257344] |
| Journal of Applied Animal Research? | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis [10.1080:09712119.2017.1335208] |
| Osteoarthritis Cartilage | Coordinated existence of multiple gangliosides is required for cartilage metabolism [Pubmed: 30471358] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166] |
| Pathobiology | Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma [Pubmed: 31129674] |
| Bone | DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion [Pubmed: 31283994] |
| Food & Function | A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese?… [] |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes [Pubmed: 32162384] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
| Nutrients | Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial [Pubmed: 31888255] |
| Nature Communications | Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13 [Pubmed: 32807790] |
| Molecular Medicine Reports | MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression [Pubmed: 32705199] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs [] |
| Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model [] | |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs [] | |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588] |
| Nutrients | Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo?… [34444810] |
| Sci Rep | Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis [Pubmed:35091584] |
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