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胰島素樣生長因子結(jié)合蛋白2(IGFBP2)多克隆抗體

DAB staining on IHC-P; Samples: Human Thyroid cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human IGFBP2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IGFBP2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IGFBP2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IGFBP2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human IGFBP2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human IGFBP2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Samples: Lane1: Human Urine; Lane2: SH-SY5Y cell lysate; Primary Ab: 0.1μg/ml Rabbit Anti-Human IGFBP2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Human Urine.
Human NB-4 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human IGFBP2 Polyclonal Antibody (Catalog PAA053Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

該抗體是針對IGFBP2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別IGFBP2。

Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

雜志	參考文獻(xiàn)
British journal of cancer	A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours. [pubmed:28664912]
Experimental and Therapeutic Medicine	Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D?LC?MS/MS [Doi: 10.3892/etm.2018.6818]
Nutrition and Cancer	Serum IGFBP2 Level Is a New Candidate Biomarker of Severe Malnutrition in Advanced Lung Cancer [Pubmed: 32286106]

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編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA053Hu01	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA053Hu61	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)活性蛋白	Cell?culture;?Activity?Assays.
UEPA053Hu61	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA053Hu01	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)多克隆抗體	WB; IHC; ICC/IF; FCM
UMAA053Hu22	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)單克隆抗體	WB; IHC; ICC; IP.
USEA053Hu	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA053Hu	胰島素樣生長因子結(jié)合蛋白2(IGFBP2)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.