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腫瘤蛋白p53(P53)多克隆抗體

Polyclonal Antibody to Tumor Protein p53 (P53)

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

編號UPAA928Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA928Hu01-腫瘤蛋白p53(P53)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/mL
且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
DAB staining on IHC-P; Sample: Porcine Colon Tissue Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P; Sample: Porcine Lymph node Tissue Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Figure 1. Western Blot; Sample: Recombinant TP53, Human.
Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Human 293T cell lysate Primary Ab: 1μg/mL Rabbit Anti-Human TP53 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Knockout Varification: Lane 1: Wild-type MCF7 cell lysate; Lane 2: TP53 knockout MCF7 cell lysate; Predicted MW: 24,30,33,34,38,39,44kDa Observed MW: 50kDa Primary Ab: 1μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Skin lysate Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對P53的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別P53。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA928Hu01	腫瘤蛋白p53(P53)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA928Hu02	腫瘤蛋白p53(P53)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA928Hu03	腫瘤蛋白p53(P53)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA928Hu01	腫瘤蛋白p53(P53)活性蛋白	Cell?culture;?Activity?Assays.
UPAA928Hu01	腫瘤蛋白p53(P53)多克隆抗體	WB; IHC; ICC/IF
UPAA928Hu02	腫瘤蛋白p53(P53)多克隆抗體	WB; IHC; FCM
ULAA928Hu71	腫瘤蛋白p53(P53)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
UMAA928Hu22	腫瘤蛋白p53(P53)單克隆抗體	WB
UMAA928Hu24	腫瘤蛋白p53(P53)單克隆抗體	WB; IHC; ICC; IP.
UMAA928Hu25	腫瘤蛋白p53(P53)單克隆抗體	WB
UMAA928Hu27	腫瘤蛋白p53(P53)單克隆抗體	WB
UMAA928Hu21	腫瘤蛋白p53(P53)單克隆抗體	WB
UMAA928Hu23	腫瘤蛋白p53(P53)單克隆抗體	WB
UFAA928Hu04	抗腫瘤蛋白p53(P53)單克隆抗體	FCM
UFAA928Hu02	抗腫瘤蛋白p53(P53)單克隆抗體	FCM
USEA928Hu	腫瘤蛋白p53(P53)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCA928Hu	腫瘤蛋白p53(P53)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMA928Hu	腫瘤蛋白p53(P53)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA928Hu01	腫瘤蛋白p53(P53)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Anticancer Research	Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233]
Microscopy Research and Technique	Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde [PubMed: 25761397]
J Nutr Biochem	Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice [PubMed: 26344777]
Biochemical and Biophysical Research Communications	Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes [Pubmed: 31331641]
Folia Biologica	Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats []
	Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… []

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