非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體
Monoclonal Antibody to Enolase 1 (ENO1)
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
- 編號UMAB449Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB449Hu02-非神經(jīng)元性烯醇化酶(ENO1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Bos taurus; Bovine (Cattle，牛)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 317 ¥ 739 ¥ 1056 ¥ 2640 ¥ 10560
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20μg/ml Mouse Anti-Human NNE Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ENO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Rat Small intestine lysate; Lane2: Bovine Cerebrum lysate; Lane3: Hela cell lysate; Lane4: PC3 cell lysate Primary Ab: 2μg/ml Mouse Anti-Human NNE Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對ENO1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ENO1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB449Hu03 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB449Hu02 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB449Hu01 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB449Hu02 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB |
| UPAB449Hu03 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB; IHC |
| UPAB449Hu01 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB |
| ULAB449Hu71 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體(生物素標(biāo)記) | WB |
| UMAB449Hu21 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu27 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu28 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu29 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu22 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB; IHC |
| UMAB449Hu23 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu24 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu25 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB |
| UMAB449Hu26 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB,IHC |
| USEB449Hu | 非神經(jīng)元性烯醇化酶(ENO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEB449Hu | 抗非神經(jīng)元性烯醇化酶抗體(Anti-ENO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCB449Hu | 非神經(jīng)元性烯醇化酶(ENO1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB449Hu | 非神經(jīng)元性烯醇化酶(ENO1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor [PubMed: 20557064] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385] |
| Journal of?Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia [Pubmed:29654921] |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis [Pubmed: 33062681] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma [Pubmed:35610567] |
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