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胱天蛋白酶1(CASP1)多克隆抗體

Polyclonal Antibody to Caspase 1 (CASP1)

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

編號(hào)UPAB592Po01
物種Sus scrofa; Porcine (Pig，豬) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB592Po01-胱天蛋白酶1(CASP1)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/mL
且適物種Homo sapiens (Human，人)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Porcine Cardiac Muscle Tissue; Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Spleen Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Pancreas Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Small intestine Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;Sample: Porcine Small intestine TissuePrimary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19)
FITC staining on IF; Sample: U2OS cell Primary Ab: 30μg/ml Rabbit Anti-Porcine CASP1 Antibody Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant CASP1, Porcine.
Western Blot; Sample: Lane1: Porcine Spleen lysate; Lane2: Porcine Lung lysate; Lane3: Raji cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification: Lane 1: Wild-type Raji cell lysate; Lane 2: CASP1 knockout Raji cell lysate; Predicted MW: 10,30,35,43,45kDa Observed MW: 42kDa Primary Ab: 5μg/ml Rabbit Anti-Porcine CASP1 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)CASP1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CASP1。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-30μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
PLoS One.	Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
Fertil Steril	Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” [PubMed: 26474737]
PLoS One	Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response. [pubmed:28704412]
Journal of?Virology	NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination [Pubmed:29386291]
Journal of Translational Medicine	Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? [Pubmed:29665864]
Reproductive?Sciences	A role for the inflammasome in spontaneous labor at term [Pubmed:27852921]
Journal?of?Virology	CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSES [Pubmed: 29386291]
ACTA?Otorhinolaryngologica Italica	NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media [Pubmed: 30676848]
American Journal of Reproductive Immunology	Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women [Pubmed: 31148259]
Taiwanese Journal of Obstetrics & Gynecology	Serum Caspase-1 levels in women with polycystic ovary syndrome [Pubmed: 32127139]
Iranian Journal of Allergy, Asthma and Immunology	Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients []
	ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES []
Norwegian Journal of development of the International Science	CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER []
LIFE SCIENCES	The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation [34560082]
Biol Reprod	The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice [34632484]
J Mol Neurosci	TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion Injury [Pubmed:35041191]
Viruses	Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network Dispersion [Pubmed:35062292]

留言咨詢

編號(hào)	適用物種：Sus scrofa; Porcine (Pig，豬)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB592Po01	胱天蛋白酶1(CASP1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAB592Po01	胱天蛋白酶1(CASP1)多克隆抗體	WB; IHC; ICC/IF
ULAB592Po71	胱天蛋白酶1(CASP1)多克隆抗體(生物素標(biāo)記)	IHC