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鈣網(wǎng)蛋白(CALR)多克隆抗體

Polyclonal Antibody to Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

編號(hào)UPAB486Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB486Hu02-鈣網(wǎng)蛋白(CALR)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度0.5mg/mL
且適物種Mus musculus (Mouse，小鼠)
應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CRT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human CRT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human CRT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant CALR, Human.
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human CRT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HL60 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human CRT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CALR Polyclonal Antibody (Catalog PAB486Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對CALR的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CALR。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UAPB486Hu61	鈣網(wǎng)蛋白(CALR)活性蛋白	Cell?culture;?Activity?Assays.
UEPB486Hu61	鈣網(wǎng)蛋白(CALR)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPB486Hu03	鈣網(wǎng)蛋白(CALR)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPB486Hu02	鈣網(wǎng)蛋白(CALR)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPB486Hu01	鈣網(wǎng)蛋白(CALR)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UCPB486Hu21	鈣網(wǎng)蛋白(CALR)卵白蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
UPAB486Hu02	鈣網(wǎng)蛋白(CALR)多克隆抗體	WB; IHC; FCM
UPAB486Hu01	鈣網(wǎng)蛋白(CALR)多克隆抗體	WB; IHC; ICC/IF; FCM
UPAB486Hu08	鈣網(wǎng)蛋白(CALR)多克隆抗體	WB; IHC; ICC; IP.
ULAB486Hu71	鈣網(wǎng)蛋白(CALR)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
UMAB486Hu22	鈣網(wǎng)蛋白(CALR)單克隆抗體	WB; IHC; ICC; IP.
USEB486Hu	鈣網(wǎng)蛋白(CALR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UCEB486Hu	鈣網(wǎng)蛋白(CALR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB486Hu	鈣網(wǎng)蛋白(CALR)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSB486Hu01	鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Journal of Investigative Dermatology	Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes [Pubmed: 23771121]
International Journal of Molecular Sciences	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC [Mdpi: Source]
The Journal of Maternal-Fetal & Neonatal Medicine	Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes [Pubmed:26953684]
journal of maternal-fetal & neonatal medicine	Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. [pubmed:28152632]
Talanta	Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling [Doi: 10.1016/j.talanta.2018.11.098]
Sci Adv	Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia [Pubmed:35171673]

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