B-細胞淋巴瘤因子2(Bcl2)多克隆抗體
Polyclonal Antibody to B-Cell Leukemia/Lymphoma 2 (Bcl2)
B-Cell CLL/Lymphoma 2
- 編號UPAA778Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA778Hu01-B-細胞淋巴瘤因子2(Bcl2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Lymphoma Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;<br/>Samples: Human Kidney Tissue; <br/>Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Bcl2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Bcl2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Tonsil Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Bcl2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;<br/>Sample: HepG2 cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Human Bcl2 Antibody<br/>Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
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Western Blot; Sample: MCF7 cell lysate Primary Ab: 0.02μg/ml Rabbit Anti-Human Bcl2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Jurkat cell lysate; Lane2: U937 cell lysate; Lane3: Porcine Pancreas lysate; Lane4: Mouse Pancreas lysate
Primary Ab: 1μg/ml Rabbit Anti-Human Bcl2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type Jurkat cell lysate; <br/>Lane 2: Bcl2 knockout Jurkat cell lysate; <br/>Predicted MW: 26kDa <br/>Observed MW: 28kDa<br/>Primary Ab: 3μg/ml Rabbit Anti-Human Bcl2 Ab<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Jurkat cell lysate; Lane3: U937 cell lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Human Bcl2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對Bcl2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別Bcl2。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA778Hu01 | B-細胞淋巴瘤因子2(Bcl2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA778Hu01 | B-細胞淋巴瘤因子2(Bcl2)多克隆抗體 | WB; IHC; ICC/IF |
| ULAA778Hu81 | B-細胞淋巴瘤因子2(Bcl2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA778Hu71 | B-細胞淋巴瘤因子2(Bcl2)多克隆抗體(生物素標記) | WB |
| UMAA778Hu22 | B-細胞淋巴瘤因子2(Bcl2)單克隆抗體 | IHC; ICC/IF |
| UMAA778Hu21 | B-細胞淋巴瘤因子2(Bcl2)單克隆抗體 | IHC; ICC/IF |
| UMAA778Hu23 | B-細胞淋巴瘤因子2(Bcl2)單克隆抗體 | IHC |
| UMAA778Hu24 | B-細胞淋巴瘤因子2(Bcl2)單克隆抗體 | IHC |
| UMAA778Hu25 | B-細胞淋巴瘤因子2(Bcl2)單克隆抗體 | IHC |
| USEA778Hu | B-細胞淋巴瘤因子2(Bcl2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA778Hu | B-細胞淋巴瘤因子2(Bcl2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA778Hu | B-細胞淋巴瘤因子2(Bcl2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233] |
| International Journal of Health Sciences | Neurotoxic effect of lead on rats: Relationship to Apoptosis [NCBI: PMC3883608] |
| Interactive cardiovascular and thoracic surgery | Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosin [Pubmed: 24327573] |
| J Physiol Biochem. | Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats [Pubmed:24752458] |
| Chem Biol Interact.? | Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities [Pubmed:25289771] |
| Pediatric Cardiology | Serum Bcl-2 Values in Children with Pulmonary Hypertension [Pubmed:25381623] |
| Histochem Cell Biol. | Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis [Pubmed:25511416] |
| PLOS ONE | Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-γ, NF-κB and Nrf2/HO-1 Signaling Pathways [PubMed: 26196679] |
| Neurochem Res | Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436] |
| European Journal of Medicinal Plants | Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms [openview:626440] |
| IJBPAS | THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS [publication:298838934] |
| Mol Neurobiol. | Neuroprotective Effect of Nanodiamond in Alzheimer's Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling [Pubmed:26897372] |
| Iranian Journal of Basic Medical Sciences | The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed rats [pubmed:27746870] |
| Scientific Reports | In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Disease [pubmed:27609215] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Journal?of?Ethnopharmacology | Amelioration?of?cardiac?dysfunction?and?ventricular?remodeling?after?myocardial?infarction?bydanhong?injection?are?critically?contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms. [pubmed:27729285] |
| European Journal of Pharmacology | Mangiferin protects against? intestinal ischemia/reperfusion-induced? liver injury:?? Involvement of PPAR-? γ, GSK-3β and Wnt/β-catenin pathway???????????????????????????????????????????????? [pubmed:28506911] |
| Journal of Applied Pharmaceutical Science | Comparative Effects of Stevia rebaudiana and Aspartame on hepato-renal function of diabetic rats: Biochemical and Histological Approaches [59b5141c458515a5b49377cf] |
| Cellular Physiology and Biochemistry | A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Rats [pubmed:28942443] |
| Biomedicine & Pharmacotherapy | Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in rats [pubmed:28554128] |
| Thromb Haemost | Distinctive expression signatures of serum microRNAs in ischaemic stroke and transient ischaemic attack patients [pubmed:28251236] |
| International Journal of Biological Macromolecules | A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats [pubmed:28709897] |
| Molecular Neurobiology | Neuroprotective Effect of Nanodiamond in Alzheimer’s Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling [pubmed:26897372] |
| Toxicology and Environmental Health Sciences | Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin [10.1007/s13530-017-0325-1] |
| Biomedicine & Pharmacotherapy | Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart [pubmed:28686977] |
| Scientific Research | Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress Disorder [paperID:82598] |
| Biomaterials | Structural optimization and additional targets identification of antisense oligonucleotide G3139 encapsulated in a neutral cytidinyl-lipid combined with a cationic lipid?… [Pubmed: 30660994] |
| PeerJ. | Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway [Pubmed: 30723634] |
| 博士論文 | INSTITUTO DE CIENCIAS BáSICAS Y PRECLíNICAS “VICTORIA DE GIRóN” DEPARTAMENTO DE HISTOLOGíA [] |
| pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034] |
| Int Immunopharmacol | Metallothionein-1 is associated with osteoarthritis disease activity and suppresses proinflammatory cytokines production in synovial cells [Pubmed: 31465913] |
| Experimental Cell Research | LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2A [Pubmed: 31505165] |
| Toxicology | LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating … [Pubmed: 32197949] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453] |
| Drug Chem Toxicol | Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascade [Pubmed: 32869669] |
| Iranian Journal of phamaceutical research | Potential anti-tumor activity of kefir-induced juglone and resveratrol fractions against Ehrlich ascites carcinoma-bearing BALB/c mice [] |
| ACS APPLIED BIO MATERIALS | Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy [] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats [] |
| Current Nutrition & Food Science | The Effect of Sodium Metabisulphite on Apoptosis in the Experimental Model of Parkinson's Disease [] |
| Life Sci | Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-|?, NF-|êB, and STAT3 crosstalk [33310046] |
| J Biochem Mol Toxicol | Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ?- [33491863] |
| Drug Des Devel Ther | Magnesium Isoglycyrrhizinate Alleviates Arsenic Trioxide-Induced Cardiotoxicity: Contribution of Nrf2 and TLR4/NF-|êB Signaling Pathway [33603344] |
| Crystals | Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent) [] |
| BIOTECHNOLOGIES | Expression of Apoptotic Markers Bcl-2 and Bax in the Vascular Wall [] |
| Pharmacol Res Perspect | [8]‐Gingerol exerts anti‐myocardial ischemic effects in rats via modulation of the MAPK signaling pathway and L‐type Ca2+ channels [34390539] |
| Ecotoxicol Environ Saf | Lycopene attenuates zearalenone-induced oxidative damage of piglet sertoli cells through the nuclear factor erythroid-2 related factor signaling pathway [34482067] |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats [34426252] |
| Environ Sci Pollut Res Int | Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity [34729716] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498] |
| Life Sci | Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and?… [Pubmed:35640776] |
| Life Sci | Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosis [Pubmed:35636581] |
| Antioxidants (Basel) | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino?… [Pubmed:35624893] |
| Experimental and Therapeutic Medicine | Nephroprotective effect of magnesium isoglycyrrhizinate against arsenic trioxide?induced acute kidney damage in mice [Pubmed:35317438] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
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