纖連蛋白(FN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Fibronectin (FN)
FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein
- 編號(hào)UKSA037Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.56-100ng/mL
- 可適靈敏度0.69ng/mL.
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 2581 ¥ 4302 ¥ 7744 ¥ 15057 ¥ 25812
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)纖連蛋白(FN)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA037Hu02 | 纖連蛋白(FN)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA037Hu03 | 纖連蛋白(FN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA037Hu61 | 纖連蛋白(FN)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA037Hu61 | 纖連蛋白(FN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA037Hu01 | 纖連蛋白(FN)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA037Hu01 | 纖連蛋白(FN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA037Hu02 | 纖連蛋白(FN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA037Hu01 | 纖連蛋白(FN)多克隆抗體 | WB; FCM |
| UPAA037Hu04 | 纖連蛋白(FN)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA037Hu02 | 纖連蛋白(FN)多克隆抗體 | WB; FCM |
| UPAA037Hu03 | 纖連蛋白(FN)多克隆抗體 | WB; IHC |
| ULAA037Hu71 | 纖連蛋白(FN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA037Hu22 | 纖連蛋白(FN)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA037Hu | 纖連蛋白(FN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA037Hu | 纖連蛋白(FN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA037Hu | 纖連蛋白(FN)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA037Hu | 纖連蛋白(FN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA037Hu01 | 纖連蛋白(FN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| American Journal of Physiology - Renal Physiology | Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase [PubMed: 19019916] |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
| Molecules. | Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. [Pubmed: 24013407] |
| Journal of Ethnopharmacology | Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy [ScienceDirect: S0378874114002414] |
| Investigative Ophthalmology & Visual Science | Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells [Pubmed: 24609629] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
| Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
| Biomedical Materials | In vivo [PubMed: 25634132] |
| American Journal of Medical and Biological Research | Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease [Ajmbr: 3] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
| journal of perinatal medicine | Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. [pubmed:27865093] |
| Chinese Journal of Contemporary Neurology and Neurosurgery | Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke [Article_en:CJFDTotal-XDJB201611014] |
| Scientific Reports | Serum fbronectin distinguishes the early stages of hepatocellular carcinoma [pubmed:28842594] |
| Molecular Medicine Reports | Pigment epithelium?derived factor protects human glomerular mesangial cells from diabetes via NOXO1?iNOS suppression [10.3892/mmr.2017.7563] |
| Cell Death & Disease | Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. [pubmed:29022912] |
| Journal of?Cellular?Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits [Pubmed:29932250] |
| Biomedicine & Pharmacotherapy | Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities [Pubmed: 30530231] |
| Advanced?Healthcare?Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
| Inflammation | TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling [Pubmed: 31111299] |
| EBioMedicine | Three kinds of corneal host cells contribute differently to corneal neovascularization [] |
| Renal Failure | Effects of autophagy on macrophage adhesion and migration in diabetic nephropathy [Pubmed: 31352855] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair [Pubmed: 31758411] |
| FASEB Journal | Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis [Pubmed: 31930571] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| International Journal of Women?ˉs Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| IBRO Neuroscience Reports | Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function [Pubmed:35586775] |
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